› 5y3z

X-ray diffraction
2Å resolution

Structure of the periplasmic domain of the MotB L119P mutant from Salmonella (crystal form 1)

Released:
DOI: 10.2210/pdb5y3z/pdb
PDB entry 5y3z coloured by chain, front view.
PDB entry 5y3z coloured by chain, side view.
PDB entry 5y3z coloured by chain, top view.
Source organism: Salmonella enterica subsp. enterica serovar Typhimurium str. LT2
Primary publication:
The Helix Rearrangement in the Periplasmic Domain of the Flagellar Stator B Subunit Activates Peptidoglycan Binding and Ion Influx.
Kojima S, Takao M, Almira G, Kawahara I, Sakuma M, Homma M, Kojima C, Imada K
Structure 26 590-598.e5 (2018)
PMID: 29576320

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-157340 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Motility protein B Chains: A, B
Molecule details ›
Chains: A, B
Length: 183 amino acids
Theoretical weight: 20.72 KDa
Source organism: Salmonella enterica subsp. enterica serovar Typhimurium str. LT2
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P55892 (Residues: 99-276; Coverage: 58%)
Gene names: STM1922, motB
Sequence domains: OmpA family

Ligands and Environments

3 bound ligands:
Ligand ARG 3 x ARG
Ligand IMD 2 x IMD
Ligand MPD 3 x MPD
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SPRING-8 BEAMLINE BL41XU
Spacegroup: P212121
Unit cell:
a: 44.13Å b: 84.8Å c: 86.83Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.193 0.19 0.221
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

15 review citations

Flagella-Driven Motility of Bacteria.
Nakamura et al. (2019)
14 more

2 mentions without citation

Structural Conservation and Adaptation of the Bacterial Flagella Motor.
Carroll et al. (2020)
1 more