5y3r

Electron Microscopy
6.6Å resolution

Cryo-EM structure of Human DNA-PK Holoenzyme

Released:
Source organism: Homo sapiens
Primary publication:
Cryo-EM structure of human DNA-PK holoenzyme.
Cell Res. (2017)
PMID: 28840859
Related structures: EMD-6803

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero hexamer (preferred)
Entry contents:
4 distinct polypeptide molecules
2 distinct DNA molecules
Macromolecules (6 distinct):
X-ray repair cross-complementing protein 6 Chain: A
Molecule details ›
Chain: A
Length: 501 amino acids
Theoretical weight: 57.62 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
UniProt:
  • Canonical: P12956 (Residues: 34-534; Coverage: 82%)
Gene names: G22P1, XRCC6
Sequence domains:
X-ray repair cross-complementing protein 5 Chain: B
Molecule details ›
Chain: B
Length: 536 amino acids
Theoretical weight: 60.97 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
UniProt:
  • Canonical: P13010 (Residues: 6-541; Coverage: 73%)
Gene names: G22P2, XRCC5
Sequence domains:
PRKDC-Helix Chain: K
Molecule details ›
Chain: K
Length: 15 amino acids
Theoretical weight: 1.08 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
DNA-dependent protein kinase catalytic subunit Chain: C
Molecule details ›
Chain: C
Length: 4119 amino acids
Theoretical weight: 468.89 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
UniProt:
  • Canonical: P78527 (Residues: 10-4128; Coverage: 100%)
Gene names: HYRC, HYRC1, PRKDC
Sequence domains:
DNA (34-MER) Chain: D
Molecule details ›
Chain: D
Length: 34 nucleotides
Theoretical weight: 10.5 KDa
DNA (36-MER) Chain: E
Molecule details ›
Chain: E
Length: 36 nucleotides
Theoretical weight: 11.04 KDa

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 6.6Å
Relevant EMDB volumes: EMD-6803
Expression system: Homo sapiens