› 5y17

X-ray diffraction
2.3Å resolution

CATPO mutant - E316F

Released:
DOI: 10.2210/pdb5y17/pdb
PDB 5y17 coloured by chain and viewed from the front.
PDB 5y17 coloured by chain and viewed from the side.
PDB 5y17 coloured by chain and viewed from the top.
Source organism: Mycothermus thermophilus
Entry authors: Karakus Yuzugullu Y, Goc G, Balci S, Pearson AR, Yorke B

Function and Biology Details

Reaction catalysed: 
2 H(2)O(2) = O(2) + 2 H(2)O
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Catalase Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 678 amino acids
Theoretical weight: 74.77 KDa
Source organism: Mycothermus thermophilus
Expression system: Escherichia coli
UniProt:
  • Canonical: M4GGR7 (Residues: 22-699; Coverage: 97%)
Sequence domains:
Structure domains:

Ligands and Environments


Cofactor: Ligand HDD 4 x HDD
1 bound ligand:
Ligand CA 10 x CA
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID30B
Unit cell:
a: 125.733Å b: 120.87Å c: 183.804Å
α: 90° β: 101.97° γ: 90°
R-values:
R R work R free
0.18 0.178 0.22
Expression system: Escherichia coli

Quick links

Citations

1 citations in other articles

Partial characterization of Bacillus pumilus catalase partitioned in poly(ethylene glycol)/sodium sulfate aqueous two-phase systems.
Yuzugullu Karakus et al. (2019)