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X-ray diffraction
1.96Å resolution

Substrate-bound Structure of G355T/Q364H mutant of a Ketoreductase from amphotericin Polyketide Synthases

Released:

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
AmphB Chains: A, B
Molecule details ›
Chains: A, B
Length: 496 amino acids
Theoretical weight: 51.48 KDa
Source organism: Streptomyces nodosus
Expression system: Escherichia coli
UniProt:
  • Canonical: Q93NW7 (Residues: 2529-3003; Coverage: 15%)
Gene name: amphB
Sequence domains: KR domain
Structure domains: NAD(P)-binding Rossmann-like Domain

Ligands and Environments

2 bound ligands:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRF BEAMLINE BL18U1
Spacegroup: P1
Unit cell:
a: 61.066Å b: 63.627Å c: 71.586Å
α: 72.67° β: 67.21° γ: 89.88°
R-values:
R R work R free
0.182 0.18 0.217
Expression system: Escherichia coli