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X-ray diffraction
1.8Å resolution

Crystal Structure of Aspergillus niger Glutamate Dehydrogenase Complexed With Alpha-ketoglutarate and NADPH

Released:
Source organism: Aspergillus niger
Entry authors: Prakash P, Punekar NS, Bhaumik P

Function and Biology Details

Structure analysis Details

Assembly composition:
homo hexamer (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Glutamate dehydrogenase Chain: A
Molecule details ›
Chain: A
Length: 460 amino acids
Theoretical weight: 49.44 KDa
Source organism: Aspergillus niger
Expression system: Escherichia coli-Thermus thermophilus shuttle vector pTRH1T
UniProt:
  • Canonical: B6V7E4 (Residues: 1-460; Coverage: 100%)
Sequence domains:

Ligands and Environments


Cofactor: Ligand NDP 1 x NDP
3 bound ligands:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE BM14
Spacegroup: R32
Unit cell:
a: 174.46Å b: 174.46Å c: 240.43Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.136 0.135 0.148
Expression system: Escherichia coli-Thermus thermophilus shuttle vector pTRH1T