5xux

X-ray diffraction
2.27Å resolution

Crystal structure of Rib7 from Methanosarcina mazei

Released:
Source organism: Methanosarcina mazei Go1
Entry authors: Yeh TM, Chen SC, Chang TH, Huang MF, Liaw SH

Function and Biology Details

Reaction catalysed:
2,5-diamino-6-(5-phospho-D-ribitylamino)pyrimidin-4(3H)-one + NAD(P)(+) = 2,5-diamino-6-(5-phospho-D-ribosylamino)pyrimidin-4(3H)-one + NAD(P)H
Biochemical function:
Biological process:
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
2,5-diamino-6-ribosylamino-4(3H)-pyrimidinone 5'-phosphate reductase Chains: A, B, C, D, E, F
Molecule details ›
Chains: A, B, C, D, E, F
Length: 240 amino acids
Theoretical weight: 26.11 KDa
Source organism: Methanosarcina mazei Go1
Expression system: Escherichia coli
UniProt:
  • Canonical: Q8PYN5 (Residues: 1-228; Coverage: 100%)
Gene name: MM_0826
Sequence domains: RibD C-terminal domain
Structure domains: Dihydrofolate Reductase, subunit A

Ligands and Environments


Cofactor: Ligand NAP 6 x NAP
No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSRRC BEAMLINE BL13C1
Spacegroup: R3
Unit cell:
a: 118.457Å b: 118.457Å c: 375.551Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.2 0.198 0.228
Expression system: Escherichia coli