› 5xtj

X-ray diffraction
2.22Å resolution

Mannanase(RmMan134A)

Released:
DOI: 10.2210/pdb5xtj/pdb
PDB entry 5xtj coloured by chain, front view.
PDB entry 5xtj coloured by chain, side view.
PDB entry 5xtj coloured by chain, top view.
Source organism: Rhizopus microsporus
Primary publication:
Structural and biochemical insights into the substrate-binding mechanism of a novel glycoside hydrolase family 134 β-mannanase.
You X, Qin Z, Li YX, Yan QJ, Li B, Jiang ZQ
Biochim Biophys Acta Gen Subj 1862 1376-1388 (2018)
PMID: 29550433

Function and Biology Details

Reaction catalysed: 
Random hydrolysis of (1->4)-beta-D-mannosidic linkages in mannans, galactomannans and glucomannans
Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-104544 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Man134A Chains: A, B
Molecule details ›
Chains: A, B
Length: 162 amino acids
Theoretical weight: 18.29 KDa
Source organism: Rhizopus microsporus
Expression system: Escherichia coli
UniProt:
  • Canonical: A0A2U8ZTY7 (Residues: 20-181; Coverage: 100%)
Sequence domains: Glycosyl hydrolase family 134

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRF BEAMLINE BL18U1
Spacegroup: P212121
Unit cell:
a: 49.259Å b: 59.35Å c: 123.304Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.173 0.173 0.211
Expression system: Escherichia coli

Quick links

Citations

2 review citations

Recent Developments in Industrial Mycozymes: A Current Appraisal.
Nath et al. (2022)
1 more