X-ray diffraction
1.06Å resolution

Crystal Structure of Transketolase in complex with TPP intermediate VIII' from Pichia Stipitis

Entry authors: Li TL, Hsu NS, Wang YL

Function and Biology Details

Reaction catalysed:
Sedoheptulose 7-phosphate + D-glyceraldehyde 3-phosphate = D-ribose 5-phosphate + D-xylulose 5-phosphate
Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Entry contents:
1 distinct polypeptide molecule
Transketolase Chain: A
Molecule details ›
Chain: A
Length: 697 amino acids
Theoretical weight: 75.05 KDa
Source organism: Scheffersomyces stipitis CBS 6054
Expression system: Escherichia coli K-12
  • Canonical: P34736 (Residues: 1-677; Coverage: 100%)
Gene names: PICST_67105, TKT, TKT1
Sequence domains:
Structure domains:

Ligands and Environments

Cofactor: Ligand T6F 1 x T6F
2 bound ligands:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSRRC BEAMLINE BL15A1
Spacegroup: C2221
Unit cell:
a: 101.104Å b: 184.934Å c: 98.918Å
α: 90° β: 90° γ: 90°
R R work R free
0.136 0.135 0.144
Expression system: Escherichia coli K-12