5xqk

X-ray diffraction
1.12Å resolution

Crystal Structure of Transketolase in complex with xylulose-5-phosphate from Pichia Stipitis

Released:
Entry authors: Li TL, Hsu NS, Wang YL

Function and Biology Details

Reaction catalysed:
Sedoheptulose 7-phosphate + D-glyceraldehyde 3-phosphate = D-ribose 5-phosphate + D-xylulose 5-phosphate
Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Transketolase Chain: A
Molecule details ›
Chain: A
Length: 697 amino acids
Theoretical weight: 75.05 KDa
Source organism: Scheffersomyces stipitis CBS 6054
Expression system: Escherichia coli K-12
UniProt:
  • Canonical: P34736 (Residues: 1-677; Coverage: 100%)
Gene names: PICST_67105, TKT, TKT1
Sequence domains:
Structure domains:

Ligands and Environments


Cofactor: Ligand T5X 1 x T5X
2 bound ligands:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSRRC BEAMLINE BL15A1
Spacegroup: C2221
Unit cell:
a: 100.709Å b: 183.724Å c: 98.856Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.123 0.123 0.134
Expression system: Escherichia coli K-12