5xpu

X-ray diffraction
2.3Å resolution

Crystal structure of MAD2L2/REV7 in complex with a CAMP fragment in a monoclinic crystal

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Mitotic spindle assembly checkpoint protein MAD2B Chain: A
Molecule details ›
Chain: A
Length: 227 amino acids
Theoretical weight: 26.1 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9UI95 (Residues: 1-211; Coverage: 100%)
Gene names: MAD2B, MAD2L2, REV7
Sequence domains: HORMA domain
Chromosome alignment-maintaining phosphoprotein 1 Chain: B
Molecule details ›
Chain: B
Length: 21 amino acids
Theoretical weight: 2.09 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q96JM3 (Residues: 325-344; Coverage: 3%)
Gene names: C13orf8, CAMP, CHAMP, CHAMP1, KIAA1802, ZNF828

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: PHOTON FACTORY BEAMLINE BL-17A
Spacegroup: C2
Unit cell:
a: 71.025Å b: 70.562Å c: 45.131Å
α: 90° β: 90.29° γ: 90°
R-values:
R R work R free
0.22 0.22 0.227
Expression system: Escherichia coli