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Anti-CRISPR proteins AcrF1/2 bound to Csy surveillance complex with a 20nt spacer crRNA backbone region

Electron Microscopy
4.2Å resolution
PDB entry 5xlp coloured by chain, front view.
PDB entry 5xlp coloured by chain, side view.
PDB entry 5xlp coloured by chain, top view.
Source organisms:
Assembly composition:
hetero hexamer (preferred)
Entry contents: 2 distinct polypeptide molecules
1 distinct RNA molecule

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Assemblies

Assembly 1 (preferred)
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Assembly Name: ACR30-35 and CRISPR-associated protein Csy3 and RNA
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Multimeric state: hetero hexamer
Accessible surface area: 64960.88 Å2
Buried surface area: 18050.65 Å2
Dissociation area: 931.53 Å2
Dissociation energy (ΔGdiss): 0.54 kcal/mol
Dissociation entropy (TΔSdiss): 11.65 kcal/mol
Symmetry number: 1
PDBe Complex ID: PDB-CPX-125822

Macromolecules

CRISPR-associated protein Csy3
Chains: C, D, E, F
Length: 342 amino acids
Theoretical weight: 37.58 KDa
Source organism: Pseudomonas aeruginosa UCBPP-PA14
Expression system: Escherichia coli 'BL21-Gold(DE3)pLysS AG'
UniProt:
  • Canonical: Q02MM1 (Residues: 1-342; Coverage: 100%)
Gene names: PA14_33310, csy1-3, csy3
Pfam: CRISPR-associated protein (Cas_Csy3)
InterPro: Type I-F CRISPR-associated protein Csy3
CRISPR-associated protein Csy3 in PDB entry 5xlp, assembly 1, front view.
CRISPR-associated protein Csy3 in PDB entry 5xlp, assembly 1, side view.
CRISPR-associated protein Csy3 in PDB entry 5xlp, assembly 1, top view.
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Uncharacterized protein AcrF1
Chain: M
Length: 78 amino acids
Theoretical weight: 8.82 KDa
Source organism: Pseudomonas phage JBD30
Expression system: Escherichia coli 'BL21-Gold(DE3)pLysS AG'
UniProt:
  • Canonical: L7P7M1 (Residues: 1-78; Coverage: 100%)
Gene name: JBD30_035
Pfam: Anti-CRISPR protein Acr30-35/AcrF1
Uncharacterized protein AcrF1 in PDB entry 5xlp, assembly 1, front view.
Uncharacterized protein AcrF1 in PDB entry 5xlp, assembly 1, side view.
Uncharacterized protein AcrF1 in PDB entry 5xlp, assembly 1, top view.
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RNA chain K
Name: crRNA with 20nt spacer sequence
Representative chains: K
Length: 48 nucleotides
Theoretical weight: 15.46 KDa
crRNA with 20nt spacer sequence in PDB entry 5xlp, assembly 1, front view.
crRNA with 20nt spacer sequence in PDB entry 5xlp, assembly 1, side view.
crRNA with 20nt spacer sequence in PDB entry 5xlp, assembly 1, top view.
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