Electron Microscopy
4.2Å resolution

Anti-CRISPR proteins AcrF1/2 bound to Csy surveillance complex with a 20nt spacer crRNA backbone region


Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
hetero hexamer (preferred)
Entry contents:
2 distinct polypeptide molecules
1 distinct RNA molecule
Macromolecules (3 distinct):
CRISPR-associated protein Csy3 Chains: C, D, E, F
Molecule details ›
Chains: C, D, E, F
Length: 342 amino acids
Theoretical weight: 37.58 KDa
Source organism: Pseudomonas aeruginosa UCBPP-PA14
Expression system: Escherichia coli 'BL21-Gold(DE3)pLysS AG'
  • Canonical: Q02MM1 (Residues: 1-342; Coverage: 100%)
Gene names: PA14_33310, csy1-3, csy3
Sequence domains: CRISPR-associated protein (Cas_Csy3)
Uncharacterized protein AcrF1 Chain: M
Molecule details ›
Chain: M
Length: 78 amino acids
Theoretical weight: 8.82 KDa
Source organism: Pseudomonas phage JBD30
Expression system: Escherichia coli 'BL21-Gold(DE3)pLysS AG'
  • Canonical: L7P7M1 (Residues: 1-78; Coverage: 100%)
Gene name: JBD30_035
crRNA with 20nt spacer sequence Chain: K
Molecule details ›
Chain: K
Length: 48 nucleotides
Theoretical weight: 15.46 KDa

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 4.2Å
Relevant EMDB volumes: EMD-6731
Expression system: Escherichia coli 'BL21-Gold(DE3)pLysS AG'