X-ray diffraction
1.77Å resolution

Crystal structure of PlsY (YgiH), an integral membrane glycerol 3-phosphate acyltransferase - the acyl phosphate form


Function and Biology Details

Reaction catalysed:
An acyl-phosphate + sn-glycerol 3-phosphate = a 1-acyl-sn-glycerol 3-phosphate + phosphate

Structure analysis Details

Assembly composition:
monomeric (preferred)
Entry contents:
1 distinct polypeptide molecule
Glycerol-3-phosphate acyltransferase Chain: A
Molecule details ›
Chain: A
Length: 201 amino acids
Theoretical weight: 21.89 KDa
Source organism: Aquifex aeolicus VF5
Expression system: Escherichia coli BL21(DE3)
  • Canonical: O66905 (Residues: 3-192; Coverage: 99%)
Gene names: aq_676, plsY
Sequence domains: Glycerol-3-phosphate acyltransferase

Ligands and Environments

1 modified residue:

Experiments and Validation Details

Entry percentile scores
X-ray source: NFPSS BEAMLINE BL18U
Spacegroup: P212121
Unit cell:
a: 46.224Å b: 65.599Å c: 84.678Å
α: 90° β: 90° γ: 90°
R R work R free
0.197 0.195 0.225
Expression system: Escherichia coli BL21(DE3)