› 5xj6

X-ray diffraction
2.37Å resolution

Crystal structure of PlsY (YgiH), an integral membrane glycerol 3-phosphate acyltransferase - the glycerol 3-phosphate form

Released:
DOI: 10.2210/pdb5xj6/pdb
PDB entry 5xj6 coloured by chain, front view.
PDB entry 5xj6 coloured by chain, side view.
PDB entry 5xj6 coloured by chain, top view.
Source organism: Aquifex aeolicus VF5
Primary publication:
Structural insights into the committed step of bacterial phospholipid biosynthesis.
Li Z, Tang Y, Wu Y, Zhao S, Bao J, Luo Y, Li D
Nat Commun 8 1691 (2017)
PMID: 29167463

Function and Biology Details

Reaction catalysed: 
An acyl-phosphate + sn-glycerol 3-phosphate = a 1-acyl-sn-glycerol 3-phosphate + phosphate
Biochemical function:
Biological process:
Cellular component:
Sequence domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-130394 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Glycerol-3-phosphate acyltransferase Chain: A
Molecule details ›
Chain: A
Length: 201 amino acids
Theoretical weight: 21.89 KDa
Source organism: Aquifex aeolicus VF5
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: O66905 (Residues: 3-192; Coverage: 99%)
Gene names: aq_676, plsY
Sequence domains: Glycerol-3-phosphate acyltransferase

Ligands and Environments

3 bound ligands:
Ligand G3P 1 x G3P
Ligand OLC 4 x OLC
Ligand PO4 1 x PO4
1 modified residue:
Ligand FME 1 x FME

Experiments and Validation Details

Entry percentile scores
X-ray source: NFPSS BEAMLINE BL19U1
Unit cell:
a: 42.148Å b: 53.772Å c: 87.688Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.221 0.219 0.263
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

3 review citations

Chemical tools for membrane protein structural biology.
Zhang et al. (2019)
2 more

1 mention without citation

Structural insights into the committed step of bacterial phospholipid biosynthesis.
Li et al. (2017)