5xg3

X-ray diffraction
3.5Å resolution

Crystal structure of the ATPgS-engaged Smc head domain with an extended coiled coil bound to the C-terminal domain of ScpA derived from Bacillus subtilis

Released:

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Chromosome partition protein Smc Chains: A, B
Molecule details ›
Chains: A, B
Length: 435 amino acids
Theoretical weight: 49.15 KDa
Source organism: Bacillus subtilis subsp. subtilis str. 168
Expression system: Escherichia coli
UniProt:
  • Canonical: P51834 (Residues: 1-219, 224-1186; Coverage: 36%)
Gene names: BSU15940, smc, ylqA
Sequence domains: RecF/RecN/SMC N terminal domain
Segregation and condensation protein A Chains: C, D
Molecule details ›
Chains: C, D
Length: 89 amino acids
Theoretical weight: 10.37 KDa
Source organism: Bacillus subtilis
Expression system: Escherichia coli
UniProt:
  • Canonical: P35154 (Residues: 167-251; Coverage: 34%)
Gene names: BSU23220, scpA, ypuG

Ligands and Environments

3 bound ligands:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: PHOTON FACTORY BEAMLINE BL-5A
Spacegroup: P212121
Unit cell:
a: 88.103Å b: 104.783Å c: 185.22Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.263 0.261 0.296
Expression system: Escherichia coli