5xey

X-ray diffraction
1.82Å resolution

Discovery and structural analysis of a phloretin hydrolase from the opportunistic pathogen Mycobacterium abscessus

Released:
Entry authors: He YX, Han JT, Jia WJ, Zhang Z

Function and Biology Details

Reaction catalysed:
Phloretin + H(2)O = phloretate + phloroglucinol
Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domain:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Phloretin hydrolase Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 279 amino acids
Theoretical weight: 31.84 KDa
Source organism: Mycobacteroides abscessus ATCC 19977
Expression system: Escherichia coli
UniProt:
  • Canonical: B1MK49 (Residues: 2-279; Coverage: 99%)
Gene name: MAB_4487c
Sequence domains: DAPG hydrolase PhiG domain

Ligands and Environments

1 bound ligand:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRF BEAMLINE BL17U
Spacegroup: P21
Unit cell:
a: 75.195Å b: 65.091Å c: 136.861Å
α: 90° β: 105.74° γ: 90°
R-values:
R R work R free
0.211 0.208 0.256
Expression system: Escherichia coli