› 5xet

X-ray diffraction
2.38Å resolution

Crystal structure of Mycobacterium tuberculosis methionyl-tRNA synthetase bound by methionyl-adenylate (Met-AMP)

Released:
DOI: 10.2210/pdb5xet/pdb
PDB 5xet coloured by chain and viewed from the front.
PDB 5xet coloured by chain and viewed from the side.
PDB 5xet coloured by chain and viewed from the top.
Source organism: Mycobacterium tuberculosis H37Ra
Primary publication:
Structural characterization of free-state and product-state Mycobacterium tuberculosis methionyl-tRNA synthetase reveals an induced-fit ligand-recognition mechanism.
Wang W, Qin B, Wojdyla JA, Wang M, Gao X, Cui S
IUCrJ 5 478-490 (2018)
PMID: 30002848

Function and Biology Details

Reaction catalysed: 
ATP + L-methionine + tRNA(Met) = AMP + diphosphate + L-methionyl-tRNA(Met)
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Methionine--tRNA ligase Chain: C
Molecule details ›
Chain: C
Length: 529 amino acids
Theoretical weight: 59.42 KDa
Source organism: Mycobacterium tuberculosis H37Ra
Expression system: Escherichia coli 'BL21-Gold(DE3)pLysS AG'
UniProt:
  • Canonical: A5U150 (Residues: 1-519; Coverage: 100%)
Gene names: MRA_1016, metG
Sequence domains:
Structure domains:

Ligands and Environments

3 bound ligands:
Ligand ME8 1 x ME8
Ligand EDO 1 x EDO
Ligand MG 5 x MG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRF BEAMLINE BL18U1
Spacegroup: R3
Unit cell:
a: 198.017Å b: 198.017Å c: 39.161Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.204 0.201 0.251
Expression system: Escherichia coli 'BL21-Gold(DE3)pLysS AG'

Quick links

Citations

2 review citations

Aminoacyl-tRNA Synthetases as Valuable Targets for Antimicrobial Drug Discovery.
Pang et al. (2021)
1 more

1 mention without citation

Structural characterization of free-state and product-state Mycobacterium tuberculosis methionyl-tRNA synthetase reveals an induced-fit ligand-recognition mechanism.
Wang et al. (2018)