› 5xdr

X-ray diffraction
2Å resolution

Crystal structure of human DEAH-box RNA helicase DHX15 in complex with ADP

Released:
DOI: 10.2210/pdb5xdr/pdb
PDB entry 5xdr coloured by chain, front view.
PDB entry 5xdr coloured by chain, side view.
PDB entry 5xdr coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
The crystal structure of human DEAH-box RNA helicase 15 reveals a domain organization of the mammalian DEAH/RHA family.
Murakami K, Nakano K, Shimizu T, Ohto U
Acta Crystallogr F Struct Biol Commun 73 347-355 (2017)
PMID: 28580923

Function and Biology Details

Reaction catalysed: 
ATP + H(2)O = ADP + phosphate
Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-128835 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
ATP-dependent RNA helicase DHX15 Chain: A
Molecule details ›
Chain: A
Length: 690 amino acids
Theoretical weight: 79.11 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: O43143 (Residues: 110-795; Coverage: 86%)
Gene names: DBP1, DDX15, DHX15
Sequence domains:

Ligands and Environments

3 bound ligands:
Ligand ADP 1 x ADP
Ligand SO4 2 x SO4
Ligand MG 1 x MG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: PHOTON FACTORY BEAMLINE BL-5A
Spacegroup: C2221
Unit cell:
a: 81.919Å b: 89.377Å c: 211.078Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.225 0.223 0.269
Expression system: Spodoptera frugiperda

Quick links

Citations

2 review citations

Distinct RNA-unwinding mechanisms of DEAD-box and DEAH-box RNA helicase proteins in remodeling structured RNAs and RNPs.
Gilman et al. (2017)
1 more

3 mentions without citation

Structural basis for DEAH-helicase activation by G-patch proteins.
Studer et al. (2020)
2 more