PDBe 5xb5

X-ray diffraction
2.23Å resolution

Crystal structure of R90A mutant of thymidylate kinase (aq_969) from Aquifex Aeolicus VF5

Released:

Function and Biology Details

Reaction catalysed:
ATP + dTMP = ADP + dTDP
Biochemical function:
Biological process:
Cellular component:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Thymidylate kinase Chains: A, B
Molecule details ›
Chains: A, B
Length: 195 amino acids
Theoretical weight: 22.32 KDa
Source organism: Aquifex aeolicus VF5
Expression system: Escherichia coli
UniProt:
  • Canonical: O67099 (Residues: 1-195; Coverage: 100%)
Gene names: aq_969, tmk
Sequence domains: Thymidylate kinase

Ligands and Environments

1 bound ligand:

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: BRUKER AXS MICROSTAR
Spacegroup: P1
Unit cell:
a: 43.01Å b: 51.84Å c: 53.64Å
α: 92.17° β: 92.67° γ: 112.83°
R-values:
R R work R free
0.243 0.24 0.286
Expression system: Escherichia coli