› 5xax

X-ray diffraction
2.9Å resolution

Parallel homodimer structures of the extracellular domains of the voltage-gated sodium channel beta4 subunit explain its role in cell-cell adhesion

Released:
DOI: 10.2210/pdb5xax/pdb
PDB entry 5xax coloured by chain, front view.
PDB entry 5xax coloured by chain, side view.
PDB entry 5xax coloured by chain, top view.
Source organism: Mus musculus
Primary publication:
Parallel homodimer structures of the extracellular domains of the voltage-gated sodium channel β4 subunit explain its role in cell-cell adhesion.
Shimizu H, Tosaki A, Ohsawa N, Ishizuka-Katsura Y, Shoji S, Miyazaki H, Oyama F, Terada T, Shirouzu M, Sekine SI, Nukina N, Yokoyama S
J Biol Chem 292 13428-13440 (2017)
PMID: 28655765

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-181706 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Sodium channel subunit beta-4 Chains: A, B
Molecule details ›
Chains: A, B
Length: 140 amino acids
Theoretical weight: 15.79 KDa
Source organism: Mus musculus
Expression system: Escherichia coli 'BL21-Gold(DE3)pLysS AG'
UniProt:
  • Canonical: Q7M729 (Residues: 30-160; Coverage: 66%)
Gene names: Gm1471, Scn4b
Sequence domains: Immunoglobulin V-set domain
Structure domains: Immunoglobulins

Ligands and Environments

1 bound ligand:
Ligand GOL 1 x GOL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SPRING-8 BEAMLINE BL44B2
Spacegroup: I23
Unit cell:
a: 131.545Å b: 131.545Å c: 131.545Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.252 0.247 0.299
Expression system: Escherichia coli 'BL21-Gold(DE3)pLysS AG'

Quick links

Citations

3 review citations

Voltage-Gated Sodium Channel β Subunits and Their Related Diseases.
Bouza et al. (2018)
2 more

2 mentions without citation

Cell-Adhesion Properties of β-Subunits in the Regulation of Cardiomyocyte Sodium Channels.
Salvage et al. (2020)
1 more