PDBe 5xaf

X-ray diffraction
2.55Å resolution

Crystal structure of tubulin-stathmin-TTL-Compound Z1 complex

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero hexamer (preferred)
Entry contents:
4 distinct polypeptide molecules
Macromolecules (4 distinct):
Tubulin alpha-1B chain Chains: A, C
Molecule details ›
Chains: A, C
Length: 451 amino acids
Theoretical weight: 50.2 KDa
Source organism: Bos taurus
Expression system: Bos taurus
UniProt:
  • Canonical: P81947 (Residues: 1-451; Coverage: 100%)
Sequence domains:
Tubulin beta-2B chain Chains: B, D
Molecule details ›
Chains: B, D
Length: 445 amino acids
Theoretical weight: 50 KDa
Source organism: Bos taurus
Expression system: Bos taurus
UniProt:
  • Canonical: Q6B856 (Residues: 1-445; Coverage: 100%)
Gene name: TUBB2B
Sequence domains:
Stathmin-4 Chain: E
Molecule details ›
Chain: E
Length: 189 amino acids
Theoretical weight: 22.13 KDa
Source organism: Rattus norvegicus
Expression system: Escherichia coli
UniProt:
  • Canonical: P63043 (Residues: 1-189; Coverage: 100%)
Gene name: Stmn4
Sequence domains: Stathmin family
TTL Protein Chain: F
Molecule details ›
Chain: F
Length: 378 amino acids
Theoretical weight: 43.55 KDa
Source organism: Gallus gallus
Expression system: Escherichia coli
UniProt:
  • Canonical: E1BQ43 (Residues: 1-378; Coverage: 100%)
Gene name: TTL
Sequence domains: Tubulin-tyrosine ligase family

Ligands and Environments

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRF BEAMLINE BL19U1
Spacegroup: P212121
Unit cell:
a: 104.568Å b: 156.509Å c: 182.078Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.208 0.206 0.257
Expression systems:
  • Bos taurus
  • Escherichia coli