5x94

X-ray diffraction
2.6Å resolution

Crystal structure of SHP2_SH2-CagA EPIYA_D peptide complex

Released:

Function and Biology Details

Reaction catalysed:
Protein tyrosine phosphate + H(2)O = protein tyrosine + phosphate
Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Tyrosine-protein phosphatase non-receptor type 11 Chains: A, B
Molecule details ›
Chains: A, B
Length: 220 amino acids
Theoretical weight: 25.01 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli 'BL21-Gold(DE3)pLysS AG'
UniProt:
  • Canonical: Q06124 (Residues: 1-220; Coverage: 37%)
Gene names: PTP2C, PTPN11, SHPTP2
Sequence domains: SH2 domain
Structure domains: SH2 domain
Cytotoxicity-associated immunodominant antigen Chains: L, M, N, O
Molecule details ›
Chains: L, M, N, O
Length: 13 amino acids
Theoretical weight: 1.52 KDa
Source organism: Helicobacter pylori F32
Expression system: Not provided
UniProt:
  • Canonical: P55980 (Residues: 966-976; Coverage: 1%)
Gene names: HP_0547, cag26, cagA, cai

Ligands and Environments

No bound ligands
2 modified residues:

Experiments and Validation Details

Entry percentile scores
X-ray source: PHOTON FACTORY BEAMLINE BL-5A
Spacegroup: P21
Unit cell:
a: 28.184Å b: 121.968Å c: 72.171Å
α: 90° β: 101.26° γ: 90°
R-values:
R R work R free
0.163 0.157 0.246
Expression systems:
  • Escherichia coli 'BL21-Gold(DE3)pLysS AG'
  • Not provided