X-ray diffraction
1.9Å resolution

Binary complex structure of a double mutant I454RA456K of o-Succinylbenzoate CoA Synthetase (MenE) from Bacillus Subtilis bound with its product analogue OSB-NCoA at 1.90 angstrom


Function and Biology Details

Reaction catalysed:
ATP + 2-succinylbenzoate + CoA = AMP + diphosphate + 2-succinylbenzoyl-CoA
Biochemical function:
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Entry contents:
1 distinct polypeptide molecule
2-succinylbenzoate--CoA ligase Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 485 amino acids
Theoretical weight: 54.22 KDa
Source organism: Bacillus subtilis subsp. subtilis str. 168
Expression system: Escherichia coli K-12
  • Canonical: P23971 (Residues: 2-486; Coverage: 100%)
Gene names: BSU30790, menE
Sequence domains:
Structure domains:

Ligands and Environments

Cofactor: Ligand S0N 4 x S0N
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRF BEAMLINE BL17U
Spacegroup: P1
Unit cell:
a: 71.95Å b: 96.48Å c: 97.66Å
α: 80.14° β: 77.81° γ: 81.17°
R R work R free
0.164 0.164 0.206
Expression system: Escherichia coli K-12