5x6e

X-ray diffraction
2.99Å resolution

Crystal structure of PrfA-DNA binary complex

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Entry contents:
1 distinct polypeptide molecule
2 distinct DNA molecules
Macromolecules (3 distinct):
Listeriolysin regulatory protein Chains: A, B, E, F, M, N
Molecule details ›
Chains: A, B, E, F, M, N
Length: 237 amino acids
Theoretical weight: 27.33 KDa
Source organism: Listeria monocytogenes
Expression system: Escherichia coli
UniProt:
  • Canonical: P22262 (Residues: 1-237; Coverage: 100%)
Gene names: lmo0200, prfA
Sequence domains: Crp-like helix-turn-helix domain
Structure domains:
DNA (29-MER) Chains: C, G, O
Molecule details ›
Chains: C, G, O
Length: 29 nucleotides
Theoretical weight: 8.85 KDa
Source organism: Listeria monocytogenes
Expression system: Not provided
DNA (28-MER) Chains: D, H, P
Molecule details ›
Chains: D, H, P
Length: 29 nucleotides
Theoretical weight: 8.97 KDa
Source organism: Listeria monocytogenes
Expression system: Not provided

Ligands and Environments

1 bound ligand:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 24-ID-E
Spacegroup: P212121
Unit cell:
a: 65.017Å b: 96.59Å c: 370.528Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.252 0.249 0.3
Expression systems:
  • Escherichia coli
  • Not provided