› 5x54

X-ray diffraction
2.3Å resolution

Crystal structure of the Keap1 Kelch domain in complex with a tetrapeptide

Released:
DOI: 10.2210/pdb5x54/pdb
PDB entry 5x54 coloured by chain, front view.
PDB entry 5x54 coloured by chain, side view.
PDB entry 5x54 coloured by chain, top view.
Source organisms:
Primary publication:
Discovery of a Kelch-like ECH-associated protein 1-inhibitory tetrapeptide and its structural characterization.
Sogabe S, Sakamoto K, Kamada Y, Kadotani A, Fukuda Y, Sakamoto JI
Biochem Biophys Res Commun 486 620-625 (2017)
PMID: 28315327

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-166298 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Kelch-like ECH-associated protein 1 Chains: A, B
Molecule details ›
Chains: A, B
Length: 291 amino acids
Theoretical weight: 31.8 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q14145 (Residues: 321-609; Coverage: 46%)
Gene names: INRF2, KEAP1, KIAA0132, KLHL19
Sequence domains: Kelch motif
Structure domains: Kelch-type beta propeller
ACE-GLU-TRP-TRP-TRP Chains: C, D
Molecule details ›
Chains: C, D
Length: 5 amino acids
Theoretical weight: 732 Da
Source organism: Escherichia phage T7
Expression system: Not provided

Ligands and Environments

1 bound ligand:
Ligand ACT 2 x ACT
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 5.0.3
Spacegroup: P212121
Unit cell:
a: 68.566Å b: 75.705Å c: 168.132Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.169 0.167 0.208
Expression systems:
  • Escherichia coli
  • Not provided

Quick links

Citations

2 review citations

Inhibitors of protein-protein interactions (PPIs): an analysis of scaffold choices and buried surface area.
Ran et al. (2018)
1 more