5x3c

Solution NMR

Solution structure of the Family 1 carbohydrate-binding module Y5A mutant with mannosylated Ser3

Released:

Function and Biology Details

Reaction catalysed:
Hydrolysis of (1->4)-beta-D-glucosidic linkages in cellulose and cellotetraose, releasing cellobiose from the non-reducing ends of the chains
Biochemical function:
Biological process:
Cellular component:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Exoglucanase 1 Chain: A
Molecule details ›
Chain: A
Length: 36 amino acids
Theoretical weight: 3.65 KDa
Source organism: Trichoderma reesei
Expression system: Not provided
UniProt:
  • Canonical: P62694 (Residues: 478-513; Coverage: 7%)
Gene name: cbh1
Sequence domains: Fungal cellulose binding domain

Ligands and Environments

1 bound ligand:
No modified residues

Experiments and Validation Details

Entry percentile scores
Chemical shift assignment: 86%
Refinement method: simulated annealing
Chemical shifts: BMR36056  
Expression system: Not provided