5x2g

X-ray diffraction
2.4Å resolution

Crystal structure of Campylobacter jejuni Cas9 in complex with sgRNA and target DNA (AGAAACC PAM)

Released:

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Entry contents:
1 distinct polypeptide molecule
2 distinct DNA molecules
1 distinct RNA molecule
Macromolecules (4 distinct):
CRISPR-associated endonuclease Cas9 Chain: A
Molecule details ›
Chain: A
Length: 835 amino acids
Theoretical weight: 96.8 KDa
Source organism: Campylobacter jejuni subsp. jejuni NCTC 11168 = ATCC 700819
Expression system: Escherichia coli
UniProt:
  • Canonical: Q0P897 (Residues: 1-480, 642-984; Coverage: 84%)
Gene names: Cj1523c, cas9
Sequence domains: RuvC endonuclease subdomain 3
Target DNA strand Chain: C
Molecule details ›
Chain: C
Length: 28 nucleotides
Theoretical weight: 8.49 KDa
Non-target DNA strand Chain: D
Molecule details ›
Chain: D
Length: 8 nucleotides
Theoretical weight: 2.44 KDa
sgRNA Chain: B
Molecule details ›
Chain: B
Length: 93 nucleotides
Theoretical weight: 30.02 KDa

Ligands and Environments

1 bound ligand:

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SLS BEAMLINE X06SA
Spacegroup: P212121
Unit cell:
a: 104.345Å b: 105.094Å c: 136.521Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.191 0.19 0.221
Expression system: Escherichia coli