5x22

X-ray diffraction
3.35Å resolution

Crystal structure of Thermus thermophilus transcription initiation complex with GpA and CMPcPP

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero nonamer (preferred)
Entry contents:
5 distinct polypeptide molecules
2 distinct DNA molecules
1 distinct RNA molecule
Macromolecules (8 distinct):
DNA-directed RNA polymerase subunit alpha Chains: A, B, K, L
Molecule details ›
Chains: A, B, K, L
Length: 315 amino acids
Theoretical weight: 35.06 KDa
Source organism: Thermus thermophilus
Expression system: Thermus thermophilus
UniProt:
  • Canonical: Q5SHR6 (Residues: 1-315; Coverage: 100%)
Gene names: TTHA1664, rpoA
Sequence domains:
DNA-directed RNA polymerase subunit beta Chains: C, M
Molecule details ›
Chains: C, M
Length: 1119 amino acids
Theoretical weight: 125.44 KDa
Source organism: Thermus thermophilus HB8
UniProt:
  • Canonical: Q8RQE9 (Residues: 1-1119; Coverage: 100%)
Gene names: TTHA1813, rpoB
Sequence domains:
DNA-directed RNA polymerase subunit beta' Chains: D, N
Molecule details ›
Chains: D, N
Length: 1524 amino acids
Theoretical weight: 171 KDa
Source organism: Thermus thermophilus HB8
UniProt:
  • Canonical: Q8RQE8 (Residues: 1-1524; Coverage: 100%)
Gene names: TTHA1812, rpoC
Sequence domains:
DNA-directed RNA polymerase subunit omega Chains: E, O
Molecule details ›
Chains: E, O
Length: 99 amino acids
Theoretical weight: 11.53 KDa
Source organism: Thermus thermophilus HB27
UniProt:
  • Canonical: Q72ID6 (Residues: 1-99; Coverage: 100%)
Gene names: TT_C1196, rpoZ
Sequence domains: RNA polymerase Rpb6
RNA polymerase sigma factor SigA Chains: F, P
Molecule details ›
Chains: F, P
Length: 443 amino acids
Theoretical weight: 50.74 KDa
Source organism: Thermus thermophilus HB27
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q72L95 (Residues: 1-423; Coverage: 100%)
Gene names: TT_C0164, rpoD, sigA
Sequence domains:
promoter DNA template strand Chains: G, Q
Molecule details ›
Chains: G, Q
Length: 21 nucleotides
Theoretical weight: 6.48 KDa
promoter DNA nontemplate strand Chains: H, R
Molecule details ›
Chains: H, R
Length: 27 nucleotides
Theoretical weight: 8.42 KDa
RNA (5'-R(*GP*A)-3') Chains: I, S
Molecule details ›
Chains: I, S
Length: 2 nucleotides
Theoretical weight: 629 Da

Ligands and Environments

3 bound ligands:

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: CHESS BEAMLINE F1
Spacegroup: P21
Unit cell:
a: 186.41Å b: 104.32Å c: 297.29Å
α: 90° β: 98.36° γ: 90°
R-values:
R R work R free
0.21 0.208 0.25
Expression systems:
  • Thermus thermophilus
  • Escherichia coli BL21(DE3)