› 5x1q

X-ray diffraction
1.6Å resolution

PpkA-294 with ATP and MnCl2

Released:
DOI: 10.2210/pdb5x1q/pdb
PDB entry 5x1q coloured by chain, front view.
PDB entry 5x1q coloured by chain, side view.
PDB entry 5x1q coloured by chain, top view.
Source organism: Serratia sp. FS14
Primary publication:
Crystal structures of the kinase domain of PpkA, a key regulatory component of T6SS, reveal a general inhibitory mechanism.
Li P, Xu D, Ma T, Wang D, Li W, He J, Ran T, Wang W
Biochem J 475 2209-2224 (2018)
PMID: 29858276

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-103705 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Protein kinase domain-containing protein Chain: A
Molecule details ›
Chain: A
Length: 302 amino acids
Theoretical weight: 34.11 KDa
Source organism: Serratia sp. FS14
Expression system: Escherichia coli
UniProt:
  • Canonical: A0A1S4NYE5 (Residues: 1-302; Coverage: 100%)
Sequence domains: Protein kinase domain

Ligands and Environments

3 bound ligands:
Ligand ATP 1 x ATP
Ligand MN 1 x MN
Ligand GOL 1 x GOL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRF BEAMLINE BL19U1
Spacegroup: P21212
Unit cell:
a: 65.898Å b: 78.315Å c: 59.188Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.181 0.179 0.212
Expression system: Escherichia coli

Quick links

Citations

1 citations in other articles

Crystal structures of PigF, an O-methyltransferase involved in the prodigiosin synthetic pathway, reveal an induced-fit substrate-recognition mechanism.
Qiu et al. (2022)