5x0t

X-ray diffraction
2.5Å resolution

Crystal structure of CD147 C2 domain in complex with Fab of its monoclonal antibody 6H8

Released:
Source organisms:
Entry authors: Lin P, Zhang M-Y, Ye S, Chen X, Yu X-L, Zhang R-G, Zhu P, Chen Z-N

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
6H8 Fab fragment heavy chain Chains: A, C
Molecule details ›
Chains: A, C
Length: 241 amino acids
Theoretical weight: 26.33 KDa
Source organism: Mus musculus
Expression system: Mus musculus
6H8 Fab fragment light chain Chains: B, D
Molecule details ›
Chains: B, D
Length: 234 amino acids
Theoretical weight: 25.91 KDa
Source organism: Mus musculus
Expression system: Mus musculus
Basigin Chains: E, F
Molecule details ›
Chains: E, F
Length: 89 amino acids
Theoretical weight: 9.84 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P35613 (Residues: 138-217; Coverage: 22%)
  • Best match: P35613-2 (Residues: 22-101)
Gene names: BSG, UNQ6505/PRO21383

Ligands and Environments

No bound ligands

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 19-ID
Spacegroup: P21
Unit cell:
a: 46.986Å b: 83.486Å c: 130.193Å
α: 90° β: 92.92° γ: 90°
R-values:
R R work R free
0.248 0.246 0.3
Expression systems:
  • Mus musculus
  • Escherichia coli