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X-ray diffraction
1.75Å resolution

Crystal structure of Mycobacterium tuberculosis VapC20 (Rv2549c), Sarcin-Ricin loop cleaving toxin

Released:

Function and Biology Details

Biochemical function:
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
23S rRNA-specific endonuclease VapC20 Chain: A
Molecule details ›
Chain: A
Length: 144 amino acids
Theoretical weight: 16.07 KDa
Source organism: Mycobacterium tuberculosis CDC1551
Expression system: Escherichia coli
UniProt:
  • Canonical: P0DMV7 (Residues: 2-131; Coverage: 99%)
Gene names: MT2626.1, vapC20
Sequence domains: PIN domain
23S rRNA-specific endonuclease VapC20 Chain: B
Molecule details ›
Chain: B
Length: 144 amino acids
Theoretical weight: 16.09 KDa
Source organism: Mycobacterium tuberculosis CDC1551
Expression system: Escherichia coli
UniProt:
  • Canonical: P0DMV7 (Residues: 2-131; Coverage: 99%)
Gene names: MT2626.1, vapC20
Sequence domains: PIN domain

Ligands and Environments

No bound ligands
1 modified residue:

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU MICROMAX-007 HF
Spacegroup: P212121
Unit cell:
a: 48.81Å b: 59.14Å c: 79.29Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.172 0.171 0.198
Expression system: Escherichia coli