5wz4

X-ray diffraction
1.78Å resolution

Crystal structure of Mycobacterium tuberculosis VapC20 (Rv2549c), Sarcin-Ricin loop cleaving toxin

Released:

Function and Biology Details

Biochemical function:
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
23S rRNA-specific endonuclease VapC20 Chains: A, B
Molecule details ›
Chains: A, B
Length: 144 amino acids
Theoretical weight: 16.19 KDa
Source organism: Mycobacterium tuberculosis H37Rv
Expression system: Escherichia coli
UniProt:
  • Canonical: P0DMV7 (Residues: 2-131; Coverage: 99%)
Gene names: MT2626.1, vapC20
Sequence domains: PIN domain

Ligands and Environments

No bound ligands
2 modified residues:

Experiments and Validation Details

Entry percentile scores
X-ray source: RRCAT INDUS-2 BEAMLINE PX-BL21
Spacegroup: P212121
Unit cell:
a: 48.82Å b: 59.28Å c: 78.62Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.176 0.175 0.212
Expression system: Escherichia coli