› 5wxy

X-ray diffraction
2.63Å resolution

Crystal structure of Microcystis aeruginosa PCC 7806 aspartate racemase in complex with L-aspartate

Released:
DOI: 10.2210/pdb5wxy/pdb
PDB 5wxy coloured by chain and viewed from the front.
PDB 5wxy coloured by chain and viewed from the side.
PDB 5wxy coloured by chain and viewed from the top.
Source organism: Microcystis aeruginosa PCC 7806
Entry authors: Cao DD, Zhou K, Jiang YL, Zhou CZ

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
McyF Chain: A
Molecule details ›
Chain: A
Length: 260 amino acids
Theoretical weight: 29.21 KDa
Source organism: Microcystis aeruginosa PCC 7806
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q9RNB4 (Residues: 1-251; Coverage: 100%)
Gene names: IPF_371, mcyF
Sequence domains: Asp/Glu/Hydantoin racemase

Ligands and Environments

1 bound ligand:
Ligand ASP 1 x ASP
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRF BEAMLINE BL18U1
Spacegroup: P3221
Unit cell:
a: 128.482Å b: 128.482Å c: 45.143Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.187 0.186 0.205
Expression system: Escherichia coli BL21(DE3)

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Citations

1 review citation

Racemases and epimerases operating through a 1,1-proton transfer mechanism: reactivity, mechanism and inhibition.
Lloyd et al. (2021)
1 other article