X-ray diffraction
2.69Å resolution

Crystal structure of a mutant insect group III chitinase complex with (GlcNAc)5 (CAD1-E647L-(GlcNAc)5 ) from Ostrinia furnacalis

Source organism: Ostrinia furnacalis
Primary publication:
The deduced role of a chitinase containing two nonsynergistic catalytic domains.
OpenAccess logo Acta Crystallogr D Struct Biol 74 30-40 (2018)
PMID: 29372897

Function and Biology Details

Reaction catalysed:
Random endo-hydrolysis of N-acetyl-beta-D-glucosaminide (1->4)-beta-linkages in chitin and chitodextrins
Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
monomeric (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecules (2 distinct):
Chitinase Chain: A
Molecule details ›
Chain: A
Length: 460 amino acids
Theoretical weight: 52.41 KDa
Source organism: Ostrinia furnacalis
Expression system: Komagataella phaffii CBS 7435
  • Canonical: U5LUV7 (Residues: 528-987; Coverage: 48%)
Sequence domains:

Ligands and Environments

Carbohydrate polymer : NEW Components: NAG
1 bound ligand:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRF BEAMLINE BL17U
Spacegroup: P41212
Unit cell:
a: 71.362Å b: 71.362Å c: 175.023Å
α: 90° β: 90° γ: 90°
R R work R free
0.208 0.205 0.237
Expression system: Komagataella phaffii CBS 7435