5wvc

X-ray diffraction
2.99Å resolution

Structure of the CARD-CARD disk

Released:

Function and Biology Details

Reaction catalysed:
Strict requirement for an Asp residue at position P1 and with a marked preference for His at position P2. It has a preferred cleavage sequence of Leu-Gly-His-Asp-|-Xaa
Biological process:
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
Non-polymer only hexamer (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Apoptotic protease-activating factor 1 Chains: A, C, E
Molecule details ›
Chains: A, C, E
Length: 95 amino acids
Theoretical weight: 10.93 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: O14727 (Residues: 1-95; Coverage: 8%)
Gene names: APAF1, KIAA0413
Sequence domains: Caspase recruitment domain
Structure domains: Death Domain, Fas
Caspase-9 subunit p35 Chains: B, D, F
Molecule details ›
Chains: B, D, F
Length: 151 amino acids
Theoretical weight: 17.41 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P55211 (Residues: 1-128; Coverage: 31%)
  • Best match: P55211-4 (Residues: 1-45)
Gene names: CASP9, MCH6
Sequence domains: Caspase recruitment domain
Structure domains: Death Domain, Fas

Ligands and Environments

1 bound ligand:
1 modified residue:

Experiments and Validation Details

Entry percentile scores
X-ray source: NSRRC BEAMLINE BL13B1
Spacegroup: P61
Unit cell:
a: 190.11Å b: 190.11Å c: 68.828Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.199 0.197 0.228
Expression system: Escherichia coli