› 5wri

X-ray diffraction
1.6Å resolution

Crystal structure of human tyrosylprotein sulfotransferase-1 complexed with PAP and C4 peptide

Released:
DOI: 10.2210/pdb5wri/pdb
PDB entry 5wri coloured by chain, front view.
PDB entry 5wri coloured by chain, side view.
PDB entry 5wri coloured by chain, top view.
Source organisms:
Primary publication:
Structural basis for the broad substrate specificity of the human tyrosylprotein sulfotransferase-1.
Tanaka S, Nishiyori T, Kojo H, Otsubo R, Tsuruta M, Kurogi K, Liu MC, Suiko M, Sakakibara Y, Kakuta Y
Sci Rep 7 8776 (2017)
PMID: 28821720

Function and Biology Details

Reaction catalysed: 
3'-phosphoadenylyl sulfate + protein tyrosine = adenosine 3',5'-bisphosphate + protein tyrosine-O-sulfate
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-130011 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Protein-tyrosine sulfotransferase 1 Chains: A, B
Molecule details ›
Chains: A, B
Length: 320 amino acids
Theoretical weight: 36.26 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: O60507 (Residues: 43-341; Coverage: 81%)
Gene name: TPST1
Sequence domains: Sulfotransferase family
Structure domains: P-loop containing nucleotide triphosphate hydrolases
ASP-PHE-GLU-ASP-TYR-GLU-PHE-ASP Chains: D, E
Molecule details ›
Chains: D, E
Length: 8 amino acids
Theoretical weight: 1.08 KDa
Source organism: synthetic construct
Expression system: Not provided

Ligands and Environments

4 bound ligands:
Ligand A3P 2 x A3P
Ligand MG 2 x MG
Ligand GOL 1 x GOL
Ligand ZN 1 x ZN
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SPRING-8 BEAMLINE BL38B1
Spacegroup: P21212
Unit cell:
a: 78.576Å b: 155.972Å c: 48.892Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.135 0.133 0.178
Expression systems:
  • Escherichia coli
  • Not provided

Quick links

Citations

2 review citations

SULFATION PATHWAYS: Insights into steroid sulfation and desulfation pathways.
Foster et al. (2018)
1 more

6 mentions without citation

From Steroid and Drug Metabolism to Glycobiology, Using Sulfotransferase Structures to Understand and Tailor Function.
Pedersen et al. (2022)
5 more