› 5wlw

X-ray diffraction
3.32Å resolution

Crystal Structure of the Human Mitochondrial Cysteine Desulfurase with active Cysteine Loop within ISCU1 active site, coordinating Zn ion. Complexed with human ISD11 and E. coli ACP1 at 3.3A.

Released:
DOI: 10.2210/pdb5wlw/pdb
PDB entry 5wlw coloured by chain, front view.
PDB entry 5wlw coloured by chain, side view.
PDB entry 5wlw coloured by chain, top view.
Source organisms:
Primary publication:
Structure and functional dynamics of the mitochondrial Fe/S cluster synthesis complex.
Boniecki MT, Freibert SA, Mühlenhoff U, Lill R, Cygler M
Nat Commun 8 1287 (2017)
PMID: 29097656

Function and Biology Details

Reaction catalysed: 
(1a) L-cysteine + [enzyme]-cysteine = L-alanine + [enzyme]-S-sulfanylcysteine
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero octamer (preferred)
PDBe Complex ID:
PDB-CPX-141284 (preferred)
Entry contents:
4 distinct polypeptide molecules
Macromolecules (4 distinct):
Cysteine desulfurase Chains: A, E
Molecule details ›
Chains: A, E
Length: 406 amino acids
Theoretical weight: 45.08 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9Y697 (Residues: 56-457; Coverage: 88%)
Gene names: HUSSY-08, NFS1, NIFS
Sequence domains: Aminotransferase class-V
LYR motif-containing protein 4 Chains: B, F
Molecule details ›
Chains: B, F
Length: 91 amino acids
Theoretical weight: 10.76 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9HD34 (Residues: 1-91; Coverage: 100%)
Gene names: C6orf149, CGI-203, ISD11, LYRM4
Sequence domains: Complex 1 protein (LYR family)
Acyl carrier protein Chains: C, G
Molecule details ›
Chains: C, G
Length: 77 amino acids
Theoretical weight: 8.51 KDa
Source organism: Escherichia coli S88
Expression system: Escherichia coli
UniProt:
  • Canonical: P0A6A8 (Residues: 2-78; Coverage: 99%)
Gene names: JW1080, acpP, b1094
Sequence domains: Phosphopantetheine attachment site
Iron-sulfur cluster assembly enzyme ISCU Chains: D, H
Molecule details ›
Chains: D, H
Length: 150 amino acids
Theoretical weight: 16.34 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9H1K1 (Residues: 38-167; Coverage: 78%)
Gene names: ISCU, NIFUN
Sequence domains: NifU-like N terminal domain
Structure domains: Sufe protein. Chain: A

Ligands and Environments


Cofactor: Ligand PLP 2 x PLP
2 bound ligands:
Ligand 8Q1 2 x 8Q1
Ligand ZN 2 x ZN
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: CLSI BEAMLINE 08ID-1
Spacegroup: P212121
Unit cell:
a: 97.529Å b: 121.241Å c: 151.484Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.2 0.196 0.255
Expression system: Escherichia coli

Quick links

Citations

20 review citations

Mitochondrial OXPHOS Biogenesis: Co-Regulation of Protein Synthesis, Import, and Assembly Pathways.
Tang et al. (2020)
19 more

11 mentions without citation

Outlining the Complex Pathway of Mammalian Fe-S Cluster Biogenesis.
Maio et al. (2020)
10 more