5wfw

X-ray diffraction
2.29Å resolution

Crystal structure of the influenza virus PA endonuclease (E119D mutant) in complex with inhibitor 10j (SRI-30026)

Released:
Entry authors: Kumar G, White SW

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
monomeric (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Polymerase acidic protein Chain: A
Molecule details ›
Chain: A
Length: 197 amino acids
Theoretical weight: 23.13 KDa
Source organism: Influenza A virus (A/California/04/2009(H1N1))
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: C3W5S0 (Residues: 1-50, 73-196; Coverage: 24%)
Gene name: PA
Sequence domains: Influenza RNA-dependent RNA polymerase subunit PA
Structure domains: Influenza RNA-dependent RNA polymerase subunit PA, endonuclease domain

Ligands and Environments

3 bound ligands:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 22-ID
Spacegroup: I422
Unit cell:
a: 90.425Å b: 90.425Å c: 133.813Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.213 0.213 0.224
Expression system: Escherichia coli BL21(DE3)