5wec

X-ray diffraction
1.56Å resolution

Structure of an alternative pilotin from the type II secretion system of Vibrio cholerae

Released:
Source organism: Vibrio cholerae O395
Entry authors: Williamson ZA, Reed RW, Korotkov KV

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Putative lipoprotein Chains: A, B
Molecule details ›
Chains: A, B
Length: 117 amino acids
Theoretical weight: 12.88 KDa
Source organism: Vibrio cholerae O395
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: A0A0H3AJJ8 (Residues: 30-143; Coverage: 80%)
Gene name: VC0395_A0582
Sequence domains: Type III secretion system lipoprotein chaperone (YscW)

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRL BEAMLINE BL9-2
Spacegroup: P212121
Unit cell:
a: 54.89Å b: 60.12Å c: 73.83Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.183 0.182 0.209
Expression system: Escherichia coli BL21(DE3)