› 5wdw

X-ray diffraction
2.3Å resolution

Crystal structure of the influenza virus PA endonuclease in complex with inhibitor 10k (SRI-30027)

Released:
DOI: 10.2210/pdb5wdw/pdb
PDB 5wdw coloured by chain and viewed from the front.
PDB 5wdw coloured by chain and viewed from the side.
PDB 5wdw coloured by chain and viewed from the top.
Source organism: Influenza A virus (A/California/04/2009(H1N1))
Primary publication:
Protein-Structure Assisted Optimization of 4,5-Dihydroxypyrimidine-6-Carboxamide Inhibitors of Influenza Virus Endonuclease.
Beylkin D, Kumar G, Zhou W, Park J, Jeevan T, Lagisetti C, Harfoot R, Webby RJ, White SW, Webb TR
Sci Rep 7 17139 (2017)
PMID: 29215062

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Polymerase acidic protein Chain: A
Molecule details ›
Chain: A
Length: 197 amino acids
Theoretical weight: 23.15 KDa
Source organism: Influenza A virus (A/California/04/2009(H1N1))
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: C3W5S0 (Residues: 1-50, 73-196; Coverage: 24%)
Gene name: PA
Sequence domains: Influenza RNA-dependent RNA polymerase subunit PA
Structure domains: Influenza RNA-dependent RNA polymerase subunit PA, endonuclease domain

Ligands and Environments

2 bound ligands:
Ligand MN 2 x MN
Ligand 8KU 1 x 8KU
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 22-ID
Spacegroup: I422
Unit cell:
a: 90.744Å b: 90.744Å c: 135.876Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.242 0.24 0.285
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

4 citation in other articles

Structural insights into the substrate specificity of the endonuclease activity of the influenza virus cap-snatching mechanism.
Kumar et al. (2021)
3 more

1 mention without citation

Crystallographically correct but confusing presentation of structural models deposited in the Protein Data Bank.
Dauter et al. (2018)