5wc9

X-ray diffraction
3.15Å resolution

Human Pit-1 and 4xCATT DNA complex

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Entry contents:
1 distinct polypeptide molecule
2 distinct DNA molecules
Macromolecules (3 distinct):
Pituitary-specific positive transcription factor 1 Chains: A, B, E, F
Molecule details ›
Chains: A, B, E, F
Length: 152 amino acids
Theoretical weight: 17.76 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P28069 (Residues: 124-273; Coverage: 52%)
Gene names: GHF1, PIT1, POU1F1
Sequence domains:
Structure domains:
DNA (5'-D(*CP*CP*AP*TP*TP*CP*AP*TP*TP*CP*AP*TP*TP*CP*AP*TP*TP*CP*GP*GP*A)-3') Chains: C, G
Molecule details ›
Chains: C, G
Length: 21 nucleotides
Theoretical weight: 6.35 KDa
Source organism: Homo sapiens
Expression system: Not provided
DNA (5'-D(*CP*CP*GP*AP*AP*TP*GP*AP*AP*TP*GP*AP*AP*TP*GP*AP*AP*TP*GP*GP*T)-3') Chains: D, H
Molecule details ›
Chains: D, H
Length: 21 nucleotides
Theoretical weight: 6.54 KDa
Source organism: Homo sapiens
Expression system: Not provided

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU MICROMAX-007 HF
Spacegroup: P21
Unit cell:
a: 66.415Å b: 97.518Å c: 85.093Å
α: 90° β: 93.65° γ: 90°
R-values:
R R work R free
0.233 0.23 0.277
Expression systems:
  • Escherichia coli
  • Not provided