› 5wb8

X-ray diffraction
3Å resolution

Crystal structure of the epidermal growth factor receptor extracellular region in complex with epigen

Released:
DOI: 10.2210/pdb5wb8/pdb
PDB entry 5wb8 coloured by chain, front view.
PDB entry 5wb8 coloured by chain, side view.
PDB entry 5wb8 coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
EGFR Ligands Differentially Stabilize Receptor Dimers to Specify Signaling Kinetics.
Freed DM, Bessman NJ, Kiyatkin A, Salazar-Cavazos E, Byrne PO, Moore JO, Valley CC, Ferguson KM, Leahy DJ, Lidke DS, Lemmon MA
Cell 171 683-695.e18 (2017)
PMID: 28988771

Function and Biology Details

Reaction catalysed: 
ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate
Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-132684 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (5 distinct):
Epidermal growth factor receptor Chains: A, D
Molecule details ›
Chains: A, D
Length: 507 amino acids
Theoretical weight: 56.52 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: P00533 (Residues: 25-525; Coverage: 42%)
Gene names: EGFR, ERBB, ERBB1, HER1
Sequence domains:
Epigen Chains: B, C
Molecule details ›
Chains: B, C
Length: 61 amino acids
Theoretical weight: 6.87 KDa
Source organism: Homo sapiens
Expression system: Drosophila melanogaster
UniProt:
  • Canonical: Q6UW88 (Residues: 49-108; Coverage: 46%)
Gene names: EPGN, UNQ3072/PRO9904

Ligands and Environments

Carbohydrate polymer : NEW Components: NAG
Carbohydrate polymer
Carbohydrate polymer : NEW Components: NAG, BMA
Carbohydrate polymer
Carbohydrate polymer : NEW Components: NAG, BMA, MAN
Carbohydrate polymer
No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 23-ID-B
Spacegroup: P4322
Unit cell:
a: 104.05Å b: 104.05Å c: 285.94Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.268 0.265 0.312
Expression systems:
  • Spodoptera frugiperda
  • Drosophila melanogaster

Quick links

Citations

40 review citations

Emerging functions of the EGFR in cancer.
Sigismund et al. (2018)
39 more

4 mentions without citation

EGFR Ligands Differentially Stabilize Receptor Dimers to Specify Signaling Kinetics.
Freed et al. (2017)
3 more