› 5wb4

X-ray diffraction
2Å resolution

Crystal structure of the TarA wall teichoic acid glycosyltransferase

Released:
DOI: 10.2210/pdb5wb4/pdb
PDB entry 5wb4 coloured by chain, front view.
PDB entry 5wb4 coloured by chain, side view.
PDB entry 5wb4 coloured by chain, top view.
Source organism: Thermoanaerobacter italicus Ab9
Primary publication:
Structure and mechanism of TagA, a novel membrane-associated glycosyltransferase that produces wall teichoic acids in pathogenic bacteria.
Kattke MD, Gosschalk JE, Martinez OE, Kumar G, Gale RT, Cascio D, Sawaya MR, Philips M, Brown ED, Clubb RT
PLoS Pathog 15 e1007723 (2019)
PMID: 31002736

Function and Biology Details

Reaction catalysed: 
UDP-N-acetyl-alpha-D-mannosamine + N-acetyl-alpha-D-glucosaminyl-diphospho-ditrans,octacis-undecaprenol = UDP + N-acetyl-beta-D-mannosaminyl-(1->4)-N-acetyl-alpha-D-glucosaminyl-diphospho-ditrans,octacis-undecaprenol
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domain:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-112543 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
N-acetylglucosaminyldiphosphoundecaprenol N-acetyl-beta-D-mannosaminyltransferase Chains: A, B, C, D, E, F, G, H
Molecule details ›
Chains: A, B, C, D, E, F, G, H
Length: 195 amino acids
Theoretical weight: 21.99 KDa
Source organism: Thermoanaerobacter italicus Ab9
Expression system: Escherichia coli 'BL21-Gold(DE3)pLysS AG'
UniProt:
  • Canonical: D3T4E0 (Residues: 1-195; Coverage: 80%)
Gene name: Thit_1850
Sequence domains: Glycosyl transferase WecG/TagA/CpsF family

Ligands and Environments

1 bound ligand:
Ligand SO4 9 x SO4
1 modified residue:
Ligand MSE 56 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 24-ID-C
Spacegroup: P21
Unit cell:
a: 77.03Å b: 107.79Å c: 89.44Å
α: 90° β: 98.29° γ: 90°
R-values:
R R work R free
0.221 0.219 0.246
Expression system: Escherichia coli 'BL21-Gold(DE3)pLysS AG'

Quick links

Citations

4 review citations

Type III Secretion Effectors with Arginine N-Glycosyltransferase Activity.
Araujo-Garrido et al. (2020)
3 more

3 mentions without citation

Improved protein structure prediction using predicted interresidue orientations.
Yang et al. (2020)
2 more