5w8t

X-ray diffraction
2.76Å resolution

Crystal structure of MERS-CoV papain-like protease in complex with the C-terminal domain of human ISG15

Released:

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Ubiquitin-like protein ISG15 Chains: B, D
Molecule details ›
Chains: B, D
Length: 79 amino acids
Theoretical weight: 8.91 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P05161 (Residues: 80-156; Coverage: 47%)
Gene names: G1P2, ISG15, UCRP
Sequence domains: Ubiquitin family
ORF1ab protein Chains: A, C
Molecule details ›
Chains: A, C
Length: 322 amino acids
Theoretical weight: 35.82 KDa
Source organism: Human betacoronavirus 2c Jordan-N3/2012
Expression system: Escherichia coli
UniProt:
  • Canonical: M4STU1 (Residues: 1482-1803; Coverage: 5%)
Gene name: ORF1ab

Ligands and Environments

3 bound ligands:

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 22-ID
Spacegroup: P43
Unit cell:
a: 86.342Å b: 86.342Å c: 223.63Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.189 0.187 0.224
Expression system: Escherichia coli