› 5w8e

X-ray diffraction
1.8Å resolution

The structure of a CoA-dependent acyl-homoserine lactone synthase, BjaI, with the adduct of SAH and IV-CoA

Released:
DOI: 10.2210/pdb5w8e/pdb
PDB entry 5w8e coloured by chain, front view.
PDB entry 5w8e coloured by chain, side view.
PDB entry 5w8e coloured by chain, top view.
Source organism: Bradyrhizobium japonicum
Primary publication:
Molecular basis for the substrate specificity of quorum signal synthases.
Dong SH, Frane ND, Christensen QH, Greenberg EP, Nagarajan R, Nair SK
Proc Natl Acad Sci U S A 114 9092-9097 (2017)
PMID: 28784791

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-166254 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Autoinducer synthase Chain: A
Molecule details ›
Chain: A
Length: 221 amino acids
Theoretical weight: 25.17 KDa
Source organism: Bradyrhizobium japonicum
Expression system: Escherichia coli

Ligands and Environments

4 bound ligands:
Ligand SXZ 1 x SXZ
Ligand CL 1 x CL
Ligand GOL 1 x GOL
Ligand ADE 1 x ADE
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 21-ID-F
Spacegroup: P3221
Unit cell:
a: 91.27Å b: 91.27Å c: 98.12Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.197 0.196 0.217
Expression system: Escherichia coli

Quick links

Citations

4 review citations

'Democratized' genomic enzymology web tools for functional assignment.
Zallot et al. (2018)
3 more

2 mentions without citation

SIFTS: updated Structure Integration with Function, Taxonomy and Sequences resource allows 40-fold increase in coverage of structure-based annotations for proteins.
Dana et al. (2019)
1 more