5w49

X-ray diffraction
2.4Å resolution

The crystal structure of human S-adenosylhomocysteine hydrolase (AHCY) bound to oxadiazole inhibitor

Released:

Function and Biology Details

Reaction catalysed:
S-adenosyl-L-homocysteine + H(2)O = L-homocysteine + adenosine
Biochemical function:
Biological process:
Cellular component:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Adenosylhomocysteinase Chains: A, B
Molecule details ›
Chains: A, B
Length: 429 amino acids
Theoretical weight: 47.44 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P23526 (Residues: 4-432; Coverage: 99%)
Gene names: AHCY, SAHH
Sequence domains:

Ligands and Environments


Cofactor: Ligand NAD 2 x NAD
2 bound ligands:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 5.0.3
Spacegroup: C2221
Unit cell:
a: 92.862Å b: 136.102Å c: 186.728Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.193 0.191 0.226
Expression system: Escherichia coli BL21(DE3)