› 5w37

X-ray diffraction
2.52Å resolution

Crystal structure of mutant CJ YCEI protein (CJ-N182C) for nanotechnology applications

Released:
DOI: 10.2210/pdb5w37/pdb
PDB 5w37 coloured by chain and viewed from the front.
PDB 5w37 coloured by chain and viewed from the side.
PDB 5w37 coloured by chain and viewed from the top.
Source organism: Campylobacter jejuni
Primary publication:
Installing Guest Molecules at Specific Sites within Scaffold Protein Crystals.
Huber TR, McPherson EC, Keating CE, Snow CD
Bioconjug. Chem. (2017)
PMID: 29232505

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assemblies composition:
monomeric (preferred)
homo tetramer
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
YceI domain-containing protein Chain: A
Molecule details ›
Chain: A
Length: 180 amino acids
Theoretical weight: 20.19 KDa
Source organism: Campylobacter jejuni
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q0PB90 (Residues: 22-190; Coverage: 100%)
Gene name: Cj0420
Sequence domains: YceI-like domain
Structure domains: Lipid/polyisoprenoid-binding, YceI-like

Ligands and Environments

2 bound ligands:
Ligand LFA 1 x LFA
Ligand SO4 2 x SO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 4.2.2
Spacegroup: P622
Unit cell:
a: 178.429Å b: 178.429Å c: 50.583Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.217 0.215 0.258
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

2 review citations

Application of Crystalline Matrices for the Structural Determination of Organic Molecules.
Cardenal et al. (2021)
1 more