PDBe 5w21

X-ray diffraction
3Å resolution

Crystal Structure of a 1:1:1 FGF23-FGFR1c-aKlotho Ternary Complex

Released:

Function and Biology Details

Reactions catalysed:
A beta-D-glucuronoside + H(2)O = D-glucuronate + an alcohol
ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
Klotho Chain: A
Molecule details ›
Chain: A
Length: 981 amino acids
Theoretical weight: 112.67 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
UniProt:
  • Canonical: Q9UEF7 (Residues: 1-981; Coverage: 97%)
Gene name: KL
Sequence domains: Glycosyl hydrolase family 1
Fibroblast growth factor 23 N-terminal peptide Chain: B
Molecule details ›
Chain: B
Length: 226 amino acids
Theoretical weight: 25.41 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9GZV9 (Residues: 25-204; Coverage: 79%)
Gene names: FGF23, HYPF, UNQ3027/PRO9828
Sequence domains: Fibroblast growth factor
Fibroblast growth factor receptor 1 Chain: C
Molecule details ›
Chain: C
Length: 226 amino acids
Theoretical weight: 25.38 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P11362 (Residues: 142-365; Coverage: 28%)
Gene names: BFGFR, CEK, FGFBR, FGFR1, FLG, FLT2, HBGFR
Sequence domains: Immunoglobulin I-set domain

Ligands and Environments

2 bound ligands:

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 24-ID-E
Spacegroup: C2
Unit cell:
a: 283.301Å b: 72.593Å c: 95.329Å
α: 90° β: 91.98° γ: 90°
R-values:
R R work R free
0.232 0.23 0.278
Expression systems:
  • Homo sapiens
  • Escherichia coli