X-ray diffraction
2.4Å resolution

Crystal structure of MBP fused activation-induced cytidine deaminase (AID) in complex with cacodylic acid

Primary publication:
AID Recognizes Structured DNA for Class Switch Recombination.
Mol. Cell 67 361-373.e4 (2017)
PMID: 28757211

Function and Biology Details

Reaction catalysed:
Cytosine in single-stranded DNA + H(2)O = uracil in single-stranded DNA + NH(3)
Biochemical function:
Cellular component:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Entry contents:
1 distinct polypeptide molecule
Maltose/maltodextrin-binding periplasmic protein; Single-stranded DNA cytosine deaminase Chains: A, B
Molecule details ›
Chains: A, B
Length: 549 amino acids
Theoretical weight: 61.66 KDa
Source organisms: Expression system: Spodoptera frugiperda
  • Canonical: Q9GZX7 (Residues: 13-181; Coverage: 85%)
  • Canonical: P0AEY0 (Residues: 27-392; Coverage: 99%)
Gene names: AICDA, AID, ECs5017, Z5632, malE
Sequence domains: Bacterial extracellular solute-binding protein

Ligands and Environments

3 bound ligands:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 24-ID-C
Spacegroup: P212121
Unit cell:
a: 36.96Å b: 179.07Å c: 188.08Å
α: 90° β: 90° γ: 90°
R R work R free
0.22 0.219 0.239
Expression system: Spodoptera frugiperda