› 5w0b

X-ray diffraction
2.61Å resolution

Structure of human TUT7 catalytic module (CM)

Released:
DOI: 10.2210/pdb5w0b/pdb
PDB entry 5w0b coloured by chain, front view.
PDB entry 5w0b coloured by chain, side view.
PDB entry 5w0b coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Multi-domain utilization by TUT4 and TUT7 in control of let-7 biogenesis.
Faehnle CR, Walleshauser J, Joshua-Tor L
Nat Struct Mol Biol 24 658-665 (2017)
PMID: 28671666

Function and Biology Details

Reaction catalysed: 
UTP + RNA(n) = diphosphate + RNA(n+1)
Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assemblies composition:
monomeric
homo trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-178454 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Terminal uridylyltransferase 7 Chains: A, B, C
Molecule details ›
Chains: A, B, C
Length: 389 amino acids
Theoretical weight: 44.72 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: Q5VYS8 (Residues: 983-1365; Coverage: 26%)
Gene names: HS2, KIAA1711, TUT7, ZCCHC6
Sequence domains:

Ligands and Environments

3 bound ligands:
Ligand ZN 1 x ZN
Ligand SO4 7 x SO4
Ligand IOD 3 x IOD
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X25
Spacegroup: P61
Unit cell:
a: 141.232Å b: 141.232Å c: 174.475Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.232 0.23 0.255
Expression system: Spodoptera frugiperda

Quick links

Citations

11 review citations

Regulation of microRNA biogenesis and its crosstalk with other cellular pathways.
Treiber et al. (2019)
10 more

3 mentions without citation

Multi-domain utilization by TUT4 and TUT7 in control of let-7 biogenesis.
Faehnle et al. (2017)
2 more